Anti-CD71 Ab and Drug treatment
For blocking CD71, RBCs were pre-treated with Anti-CD71 Ab (abcam) or
Rabbit IgG Ab
(Beyotime)
for 1 h, and then infected with PEDV for 1 h at 37 °C. For inhibition of
clathrin-mediated endocytosis,
RBCs
were pre-treated with the respective doses of
chlorpromazine
(CPZ) (Selleck) for 2 h and then infected with PEDV in RPMI 1640
medium
containing CPZ for 1 h at 37 °C. After infection, RBCs were collected
and counted to detect viral titer.