Anti-CD71 Ab and Drug treatment
For blocking CD71, RBCs were pre-treated with Anti-CD71 Ab (abcam) or Rabbit IgG Ab (Beyotime) for 1 h, and then infected with PEDV for 1 h at 37 °C. For inhibition of clathrin-mediated endocytosis, RBCs were pre-treated with the respective doses of chlorpromazine (CPZ) (Selleck) for 2 h and then infected with PEDV in RPMI 1640 medium containing CPZ for 1 h at 37 °C. After infection, RBCs were collected and counted to detect viral titer.