Profile of potentials N and O-glycosylated sites
Analysis of potentials N- and O-glycosylated sites showed distinct patterns of glycosylation sites among CAF isolates.
Among RSV type A isolates a new N-glycosylated site (NTT code) appeared at amino acid position 245 of isolates of NA1 genotypes. The other N-glycosylated sites (position 259 and 302 of NA1 genotype, fig. S3; position 247 and 318 of ON1 genotype, fig. S4) were identical to the prototype strains.
In RSV type B isolates, a new N-glycosylated site (NTT code) appeared in the duplicate area at amino acid position 273 (Fig. S5).
The O-glycosylation profile was similar when 22 amino acid sequences for the ON1 genotype G protein and 3 amino acids for the CAF NA1 genotype G protein were analyzed. The O-glycosylation profile predicted (402 sites for ON1 and 102 sites for NA1) serine and threonine and would be O-glycosylated with a score of 0.5-0.7.
Analysis of O-glycosylated amino acid sites of 7 strains of RSV-B predicted up to 77 potential O-glycosylated sites of serine and threonine residues with a score of 0.5-0.7.