Measurement of cytokine levels in BALF and SHS
The concentrations of interleukin
(IL)-4, IL-13,
interferon
(IFN)-γ, and IL-10 in BALF and SHS
were measured by sandwich
enzyme-linked immunosorbent assay
(ELISA), in accordance with the
manufacturer’s instructions (eBioscience Co., San Diego, CA, USA).
Values were interpolated from standard curves using recombinant
cytokines and expressed in pg/mL.