Table Captions
Table S1. The relative abundance of Prokaryote and fungi at phylum level under different water table treatments in short-term and long-term drainage peatlands.
Table S2. The average GHG emission rate and temperature sensitivity (Q10) of three different water table treatments in short- and long-term drainage peatlands under 8 °C and 18 °C.
Table S3. Soil DOC and TDN concentration variations under different water table (WT) treatments in short-term and long-term drainage peatlands.
Table S4. Data on soil and plant properties in short-term and long-term drained peatlands.
Figure captions
Figure 1. Map of sampling site (Ruokeba) in Zoige Plateau peatland
Figure 2. The compositions and structures of prokaryotic (a,c) and fungal (b,d) in peatlands with different drainage age and water table levels. S: Short-term drainage sites, L: Long-term drainage sites, S2 and L2: low water table treatment (LWT), S10 and L10: intermediate water table treatment (IWT), S50 and L50: high water table treatment (HWT).
Figure 3. Microbial biomarkers and predicted functional profiles of prokaryote with differen water table levels. (a) Top 14 prokaryotic families were identified by using random-forest classification of the relative abundance of prokaryote with different water table levels. (b) Heatmap of the relative abundance of biomarker families in the individual samples. (c) The differeces prokaryotic function in the FaProTax database for different water table treatments. S: Short-term drainage site, L: Long-term drainage site, S2 and L2: low water table treatment (LWT), S10 and L10: intermediate water table treatment (IWT), S50 and L50: high water table treatment (HWT).
Figure 4. The average GHG emission rate during 35d incubation in short- and long-term drainage peatlands with three different water table treatments under 8 °C and 18 °C. (a) CO2 emissions; (b) CH4 emissions; (c) N2O emissions. Significance level: *p< 0.05, **p < 0.01, or ***p< 0.001. S: Short-term drainage site, L: Long-term drainage site, S2 and L2: low water table treatment (LWT), S10 and L10: intermediate water table treatment (IWT), S50 and L50: high water table treatment (HWT).
Figure 5. Variation of DOC (a) and TDN (b) concentration of three different water table treatments in short- and long-term drainage peatlands under 8 °C and 18 °C.Significance level: *p < 0.05, **p < 0.01, or ***p < 0.001. S: Short-term drainage site, L: Long-term drainage site, S2 and L2: low water table treatment (LWT), S10 and L10: intermediate water table treatment (IWT), S50 and L50: high water table treatment (HWT).
Figure 6.Relationship between the average GHG emission and DOC(a, c, e) and TDN concentration (b, d, f). Grey circle represents at 8 °C, and red circle represents at 18 °C.
Figure 7. Spearman’s correlation analysis between the dominant microbial phyla and GHG emissions, DOCconcentration and TDN concentration. Grey font indicates fungal phyla, and black font indicates prokaryotic phyla. Significance level: *p < 0.05, **p < 0.01, or ***p < 0.001.
Figure 8. The impacts of water table, drainage age, soil properties and soil prokaryotic and fungal communities on GHG emissions as estimated using the structural equation modeling.( a) CO2emissions; (b) CH4 emissions; (c) N2O emissions. Standardized path coefficients are listed beside each path (Red and blue arrows represent positive and negative relationships, black double arrows represent covariant correlation; Paths with insignificant coefficients are represented using gray lines; *p < 0.05, **p< 0.01, or ***p < 0.001). R2 values indicate the strength of explanation by independent variables. The prokaryotic and fungal diversities are represented using Shannon indexes, and the prokaryotic and fungal communities are represented using NMDS of the Bray-Curtis distance matrix.
Figure S1. Water table data at different distances from short- and long-term drainage ditches in recent three growing seasons (2016-2018). (a) The fluctuation of water table depth (WTD); (b) Average water table depths. S: Short-term drainage peatlands, L: Long-term drainage peatlands, S2 and L2: low water table treatment (LWT), S10 and L10: intermediate water table treatment (IWT), S50 and L50: high water table treatment (HWT).
Figure S2. Variation of GHG emission rate of soil from three different water table treatments in short- and long-term drainage peatlands under 8 °C and 18 °C.(a) CO2 emission rate at 8°C; (b) CH\sout4 emission rate at 8°C; (c) N2O emission rate at 8°C; (d) CO2 emission rate at 18°C; (e) CH4 emission rate at 18°C; (f) N2O emission rate at 18°C.
Figure S3. Temperature sensitivity (Q10) value variations from three different water table treatments in short- and long-term drainage peatlands. (a) CO2; (b) CH4; (c) N2O. Significance level: *p < 0.05, **p < 0.01, or ***p< 0.001. S: Short-term drainage site, L: Long-term drainage site, S2 and L2: low water table treatment (LWT), S10 and L10: intermediate water table treatment (IWT), S50 and L50: high water table treatment (HWT).
Figure S4. Spearman’s correlation analysis between GHG emissions and soil properties, soil prokaryotic and fungal communities. Significance level: *p< 0.05, **p < 0.01, or ***p< 0.001.