2.4 Subcellular localization of NtMYB12a-GFP fusion protein
The subcellular localization of NtMYB12a was analyzed in tobacco
protoplasts as described previously (Yoo,
Cho, & Sheen, 2007). The recombinant 35S:NtMYB12a-GFP and
35S: GFP plasmid was introduced into tobacco protoplasts obtained
from leaf tissues using polyethylene glycol-mediated transformation. The
fluorescent signal was captured 16-20 h after transformation with a
confocal laser scanning microscope (ZEISS LSM 700, Germany). The nuclear
localized marker protein (NLS) was as described in the previous
reference (Shcherbo et al., 2007).