2.9 GR induction
Tobacco seeds harboring NtMYB12a-GR fusion protein were surface
sterilized with 6% of sodium hypochlorite, and germinated on MS medium.
15 day seedlings were transferred to new mediums containing DMSO
(control), DEX (10 μM), CYC (5 μM), and DEX+CYC, respectively. The
seedlings were collected at 1 h, 4 h, and 8 h after treatment, and used
for RNA extraction and gene expression level analyses.