2.7| Statistical analysis
All rearing experiments to evaluate survival rates were performed at least in triplicate, and Na+, K+, and osmotic concentrations were measured in at least three crabs in each group. Error bars in the figures show standard errors. Mean values comparisons among more than three groups were performed using analysis of variance (ANOVA), followed by Williams’ test, Student’s t -test with Bonferroni correction, or Tukey’s test. Mean values comparisons between two groups were performed by Student’s t -test. Results were considered statistically significant if the P value was less than 0.05.