Comparison of the two methods for analysis of carrier frequency and predicted genetic prevalence
The carrier frequency for a variant (CFV) was calculated based on the allele frequency of each heterozygous PLPV except homozygous carriers. The CFG and pGPGwere analyzed using two different methods as described in the methods section for every gene in each ethnic group, and the CFGand pGPG values calculated by the two methods were compared. Notably, linear regression analysis showed a perfect fit: R2=1 (y = 0.00001814 + 0.990x) for CFG(Figure 1A) and R2=1 (y = 0.0000001347 + 0.961x) for pGPG (Figure 1B).