3.1 Cut-off of iELISA
Assuming the molecular detection of MYXV/ha-MYXV as reference diagnostic test, the cut-off RI10 = 6.1 yielded an estimated positive predictive value of 96.4% (CI95% 82.6 – 98.0%), a specificity of 99.1% (CI95% 95.2 – 100%) and a sensitivity of 52.9% (CI95% 38.5 -67.1%). The area under the ROC curve was estimated at 79.2% (CI95% 69.7 – 88.7%). The finite mixture model estimated the seronegative subgroup as a normal distribution of RI10 with mean = 0.803 and standard error = 1.714. Under the assumption of normality of the log transformed RI10 seronegative data, the cut-off RI10=6.1 yielded an estimated specificity of 99.9%.