Quantification of Cytokines with Luminex
At the end of the experiment, blood was collected into an EDTA containing Eppendorf tube and then centrifuged (2000 rpm 10 min) to obtain plasma samples.
Luminex technology (Mouse High Sensitivity T Cell Magnetic Bead Panel (MHSTCMAG-70K); Merck GmbH, Darm stadt, Germany) was used to measure IFNγ, IL-1β, IL-6, IL-17, and TNFα in the plasma. All quantifications were done according to the protocols provided by the manufacturer.