Aphid fecundity
Wild type as well as rms3-1 and rms4-1 pea mutants were
germinated and transplanted in the same media and growth conditions as
described above. In order to obtain A. pisum nymphs of the same
age, adult A. pisum was placed on WT pea for 24 h and allowed to
produce nymphs. One of these nymphs were then placed a 5-day old plant
from each of the genotypes, directly after transplantation. The aphids
were contained on the pea plants using 07ZL flowerpot cages
(Insectopia), for 15 days while the aphids were allowed to reproduce
(Figure 7). Ten replicates were performed for each pea genotype at
either ambient or elevated CO2. The number of aphids
present on each plant was then determined.