Figure 2. Cell types derived from the olfactory
neuroepithelium (ON) of cannabis users and control subjects . Cells
cultured to passage 6 were incubated for 24 hours under proliferative
conditions. Representative immunofluorescence images and quantification
of the different cell populations derived from a control subject and a
cannabis user. Specific cell populations are immunolabelled in green or
red and the nuclei are stained with DAPI. The quantification of the
cells in each population is represented relative to the total cells
(DAPI+): (A-C) Mesenchymal
CD105+ cells; (D-F) Nestin+neural progenitor cells; (G-I) Glial-like
GFAP+ cells. (J-L) Immature β-III-Tubulin
neurons; (M-O) Immature GAP43+ neurons. Scale
bar = 50μm. * p < 0.05; ** p < 0.001
(Student’s t-test , n=5).