2.7. Cell size and shape measurement
Neuronal microtubules were identified by β-III-Tubulin immunostaining,
as described above and in triplicate cultures for each sample. A total
of 100 cells were assessed per subject from 15 random microscopy fields.
The area and perimeter occupied by microtubules was defined and measured
with the ImageJ software (USA). The formula used to assess roundness
was: (perimeter2)/(4*pi*area). A roundness value of
1.0 indicates a perfect circle, and higher values indicate oblong and
non-circular objects (DeCoster et al., 2010).