2.11. Wound healing assay
The assay was performed as indicated in the datasheet for the “Wound
Healing Assay” (Ibidi, Germany). A 70 µl suspension of a 3 x
105 cells was added into each well of the 2 Well
Culture-Insert and after 24 hours, when the cell monolayer was
confluent, the 2 Well Culture-Insert was removed and fresh
medium was added. At this point (0 hours) a wound was made in the
culture, which was free of cells, and images were then taken at 0, 24
and 48 hours in order to quantify the number of cells migrating into the
wound and the percentage closure. Each sample was studied in triplicate.