2.11. Wound healing assay
The assay was performed as indicated in the datasheet for the “Wound Healing Assay” (Ibidi, Germany). A 70 µl suspension of a 3 x 105 cells was added into each well of the 2 Well Culture-Insert and after 24 hours, when the cell monolayer was confluent, the 2 Well Culture-Insert was removed and fresh medium was added. At this point (0 hours) a wound was made in the culture, which was free of cells, and images were then taken at 0, 24 and 48 hours in order to quantify the number of cells migrating into the wound and the percentage closure. Each sample was studied in triplicate.