Figure 2. Cell types derived from the olfactory neuroepithelium (ON) of cannabis users and control subjects . Cells cultured to passage 6 were incubated for 24 hours under proliferative conditions. Representative immunofluorescence images and quantification of the different cell populations derived from a control subject and a cannabis user. Specific cell populations are immunolabelled in green or red and the nuclei are stained with DAPI. The quantification of the cells in each population is represented relative to the total cells (DAPI+): (A-C) Mesenchymal CD105+ cells; (D-F) Nestin+neural progenitor cells; (G-I) Glial-like GFAP+ cells. (J-L) Immature β-III-Tubulin neurons; (M-O) Immature GAP43+ neurons. Scale bar = 50μm. * p < 0.05; ** p < 0.001 (Student’s t-test , n=5).