Singleplex PCR test
Singleplex PCR reactions of the 45 loci in ten species representing all
major lineages of catarrhines were run on agarose gels and resulted, for
all loci and species, in PCR products within the expected size range
with no signs of amplifying any off-target PCR products (data not
shown). Thus, locus-specificity and universal applicability of our
primer set to catarrhine primates was indicated.