2.4 Cytokine assays
Cytokines in bone marrow and
peripheral blood were detected by multiplex bead-based flow cytometric
immunoassays. Multiplex assay kits for IL-5, TNF-α, IL-2, IL-6, IL-1β,
IL-10, IFN-γ, IL-8, IL-17 and IL-12 were purchased from RAISECARE in
China. Above cytokines were tested in plasma samples obtained by
centrifugation of heparinized bone marrow or peripheral blood from AA
and control children.