Figure legends
FIGURE 1. CD3+, CD4+ and
FoxP3+ cells near endosteum detected by
immunohistochemistry. The numbers of CD3+,
CD4+ and FoxP3+ cells all decreased
in AA patients.
FIGURE 2. Tregs decreased in IP sites .
FoxP3+/CD4+(A) and
FoxP3+(B) were analyzed in normal control, MDS and AA.
Both FoxP3+/CD4+ and
FoxP3+ cells in AA were lower than that in normal
control and MDS.
FIGURE 3. Proportion of Th1, Th2 and Th17 cells in bone marrow.A. The percentages of Th1, Th2 and Th17 in normal control and AA
measured by flow cytometry. B. Compared with the normal control group,
the percentage of Th1 and Th17 in AA group increased significantly.
FIGURE 4. Expression of TNF-α, IFN-γ and IL-17 among bone marrow
aspirates mononuclear cells. A. Compared with the normal control group,
TNF, IFN- γ and IL-17 significantly increased in bone marrow of aplastic
anemia. B. Determination of TNF-α, IFN-γ and IL-17 in peripheral blood
by Flow cytometry, there were no differences between normal control and
AA.
FIGURE 5. Bone marrow hematopoietic stem cell subsets: LT-HSCs
and ST-HSCs. A. The content of LT-HSCs and ST-HSCs in normal and AA
detected by flow cytometry. B. LT-HSCs and ST-HSCs in bone marrow of
aplastic anemia, especially the LT-HSCs, were significantly lower than
those in the control group.