Figure legends
FIGURE 1. CD3+, CD4+ and FoxP3+ cells near endosteum detected by immunohistochemistry. The numbers of CD3+, CD4+ and FoxP3+ cells all decreased in AA patients.
FIGURE 2. Tregs decreased in IP sites . FoxP3+/CD4+(A) and FoxP3+(B) were analyzed in normal control, MDS and AA. Both FoxP3+/CD4+ and FoxP3+ cells in AA were lower than that in normal control and MDS.
FIGURE 3. Proportion of Th1, Th2 and Th17 cells in bone marrow.A. The percentages of Th1, Th2 and Th17 in normal control and AA measured by flow cytometry. B. Compared with the normal control group, the percentage of Th1 and Th17 in AA group increased significantly.
FIGURE 4. Expression of TNF-α, IFN-γ and IL-17 among bone marrow aspirates mononuclear cells. A. Compared with the normal control group, TNF, IFN- γ and IL-17 significantly increased in bone marrow of aplastic anemia. B. Determination of TNF-α, IFN-γ and IL-17 in peripheral blood by Flow cytometry, there were no differences between normal control and AA.
FIGURE 5. Bone marrow hematopoietic stem cell subsets: LT-HSCs and ST-HSCs. A. The content of LT-HSCs and ST-HSCs in normal and AA detected by flow cytometry. B. LT-HSCs and ST-HSCs in bone marrow of aplastic anemia, especially the LT-HSCs, were significantly lower than those in the control group.