Lymphocyte reconstitution
Serial ALC assessments showed a gradual increase from D+30 to D+180. Median counts reached 634/µL (8-3 133) on D+30, 1 022/µL (133-5 503) on D+100 e 1 541/µL (187-14 432) on D+180, representing 82%, 89% and 90% of recovered patients, respectively. Difference in ALC according to the donor was significant on D+30 (Fig. 1).
Flow cytometry analysis on D+100 showed the highest CD3+CD8+ median count (336/µL, 8-3 569), followed by CD3+CD4+ (154/µL, 8-689) and CD16+CD56+ (130/µL, 14-1 184) while CD19+ had the lowest (56/µL, 0-727). CD19+ had a slow reconstitution and a significant difference according to the donor, as haploidentical HSCT (haplo-HSCT) had a median of 23/µL whereas 69/µL for MSD and 72/µL for unrelated donor (Fig. 2).