3.6 | Comparison of anti-FcεRI stimulation versus anti-IgE stimulation
Because we observed a paradoxical surface-bound IgE status in AD basophils, we refocused on the differences in two other stimulations of FcεRI. Specifically, we compared the response ratios after anti-FcεRI stimulation and anti-IgE stimulation in AD patients and HCs. In HCs, the CD203c response ratio for anti-FcεRI/baseline was lower than anti-IgE/baseline (P < .001) (Figure 6 B). This observation was also found in our previous report.33 In contrast, the CD203c response ratio in AD patients was higher for anti-FcεRI/baseline than for anti-IgE/baseline (P = .02) (Figure 6 A). Thus, the responsiveness of basophils to anti-FcεRI stimulation and anti-IgE stimulation was opposite between AD patients and HCs. From another point of view, the responsiveness of basophils in AD patients was maintained for anti-FcεRI stimulation, but reduced for anti-IgE stimulation.