3.3 | Analysis of basophil response markers including CD203c and CD63
To determine the baseline basophil status in AD patients, we analyzed basophil activation status using the basophil response markers CD203c and CD63 in both AD patients and HCs. Unlike a previous report,8 AD patients had a higher baseline CD203c (P < .001) and a lower CD203c response ratio with anti-IgE stimulation (P < .001) than HCs (Figure 2 A, B). Baseline CD63 in AD patients was higher than that in HCs (P< .001) (Figure 2 C). CD63 response ratio with anti-IgE stimulation was lower in AD patients than in HCs (P < .001) (Figure 2 D). Thus, CD203c with no stimulation and with anti-IgE stimulation on AD basophils showed a similar expression pattern to CD63. These data suggest that AD basophils were activated spontaneously with no stimulation and exhibited low responsiveness to anti-IgE stimulation.