3.3 | Analysis of basophil response markers including
CD203c and CD63
To determine the baseline basophil status in AD patients, we analyzed
basophil activation status using the basophil response markers CD203c
and CD63 in both AD patients and HCs. Unlike a previous
report,8 AD patients had a higher baseline CD203c
(P < .001) and a lower CD203c response ratio with
anti-IgE stimulation (P < .001) than HCs (Figure 2 A,
B). Baseline CD63 in AD patients was higher than that in HCs (P< .001) (Figure 2 C). CD63 response ratio with anti-IgE
stimulation was lower in AD patients than in HCs (P <
.001) (Figure 2 D). Thus, CD203c with no stimulation and with anti-IgE
stimulation on AD basophils showed a similar expression pattern to CD63.
These data suggest that AD
basophils were activated spontaneously with no stimulation and exhibited
low responsiveness to anti-IgE stimulation.