Testing
The Digene HC2 NA Collection Device was used for both self- and
physician-collected sampling. The self-collected and clinician-collected
swabs were transported to and tested at the PHRII laboratory. Both the
swabs were tested on the digene Hybrid Capture 2 HPV DNA Test,
Qiagen platform that used an RNA probe cocktail that detects 13
high-risk HPV types (16/18/31/33/35/39/45/51/52/56/ 58/59/68). To
prevent false negatives caused by gene deletions, the digene HC2
HPV DNA Test uses full genome RNA probes complementary to HPV DNA,
specific antibodies, signal amplification, and chemiluminescent
detection. Women were given verbal and pictorial instructions prior to
testing to use a cervical brush, which was then swirled and stored in
the PreservCyt Solution.