Mitogenome Assembly and Annotation
The N. riversi mitochondrial genome was assembled using the
SMART2 software package (Alqahtani & Măndoiu 2019), based on one
quarter of the Illumina pair-end short read genomic data. We confirmed
the gene order by mapping long-read data to this assembly in
Geneious Prime 2019 (Biomatters Ltd.). The mitochondrial genome
was then annotated using MITOS (Bernt et al. 2013) and coding
regions were subsequently refined manually in Geneious with
reference to published beetle mitochondrial genomes (Timmermans &
Vogler 2012). We compared the resulting annotated genome to a published
mitochondrial genome for Nebria brevicollis (KT876906; Linardet al. 2016) and to the gene order of other Carabidae
(López-López & Vogler 2017). The mitochondrial genome assembly has been
made publicly available on NCBI (Accession: MW244066).