Transcriptome and Mitochondrial Genome Assemblies
The assembled transcriptome was 297.65 Mb, encompassing 129,804 genes
and 201,715 transcripts (Table 2) . Based on blast searches
against full length reference proteins in the UniProt-SwissProt
database, the transcriptome had 3,985 transcripts with
>80% and 2,869 transcripts >90% alignment
coverage on reference proteins. The BUSCO assessment of 2,442 single
copy orthologs shows 97.4% completeness (complete and single copy
=19.2%, complete and duplicated = 78.2%, fragmented = 1.6%, and
missing = 1.0%). The high number of duplicated genes is due to the
presence of several isoforms in the trinity output. When only the
longest isoform per gene is used, the transcriptome size is 100.02 Mb
(contig N50 = 3,991 bp, with an average and median contig length of
1,475 and 489 bp, respectively).
The mitochondrial genome of N. riversi is 15.8 Kb, with a full
complement of 37 genes (Figure S1 and Table S2 ). This
is substantially smaller than the published mitochondrial genome ofN. brevicollis (21.2 Kb), which has a large expansion of
non-coding DNA near the replication origin. However, the mitochondrial
genome of N. riversi is similar in size to the majority of
Coleopteran mitochondrial genomes, and the gene order is largely
conserved, except for a novel inversion with respect to other Carabidae
that encompasses the nad2, trnI, trnM and trnW genes
adjacent to the replication origin.