Mitogenome Assembly and Annotation
The N. riversi mitochondrial genome was assembled using the SMART2 software package (Alqahtani & Măndoiu 2019), based on one quarter of the Illumina pair-end short read genomic data. We confirmed the gene order by mapping long-read data to this assembly in Geneious Prime 2019 (Biomatters Ltd.). The mitochondrial genome was then annotated using MITOS (Bernt et al. 2013) and coding regions were subsequently refined manually in Geneious with reference to published beetle mitochondrial genomes (Timmermans & Vogler 2012). We compared the resulting annotated genome to a published mitochondrial genome for Nebria brevicollis (KT876906; Linardet al. 2016) and to the gene order of other Carabidae (López-López & Vogler 2017). The mitochondrial genome assembly has been made publicly available on NCBI (Accession: MW244066).