Methods:
Collection of medicinal
plant
Berberis lycium Royle plant was collected from Chinari, District
Jhelum valley, Azad Kashmir, Pakistan. Root bark was separated and
washed with distilled water. Shade dried root bark was crushed into fine
powder.
Preparation of plant
extract
Ten gram of powder was boiled with 100 ml distilled water. After cooling
extract (BLR extract) was filtered using filter paper and concentrated
using rotary evaporator. Plants extract was dried in vacuum oven at 40°C
and finally used for evaluation of antidiabetic activity.
Phytochemical
Screening
The aqueous extract of BLR root bark was screened out for the presence
of different phytochemicals.
Chemicals and Drugs: Glibenclamide was purchased from a local
drug store and alloxan (Sigma Aldrich, USA) from chemical center
Anarkali, Lahore.
In Vitro antidiabetic
activity
α-amylase inhibition assay was completed using the DNSA method (Miller,
1959) and α glucosidase inhibition activity method of Kim et al., (2005)
with some modification.
In vivo antidiabetic
activity
Animal
Management
Mice of various groups were kept in polypropylene cages. Normal
laboratory pellet feed as well as drinking water ad libitum was
provided to mice. Animal house temperature was retained at 22 ± 3 °C. 12
h light/12 h dark cycle was retained. Oral feeding needle (18-gauge) was
used for oral dosing.