Biochemical Determinations
After 28 days treatment blood was collected directly from heart and mice were sacrificed for different biochemical analysis and histology. Blood glucose (at 7th, 14th, 21st and 28th day), lipid profile (Cholesterol, triglycerides, HDL-C, LDL-C and VLDL-C), LFT (ASAT, ALAT, ALP, bilirubin and total protein), RFT (Albumin, creatinine and urea), antioxidant activity (SOD, CAT and LPO) and hematological parameters were analyzed. Histopathological studies were also carried out. See (figure 1) for complete research plan.

Arithmetical analysis

GraphPad prism (version 5.0) was used for arithmetical studies. All values were expressed as mean±standard error of mean. Statistical difference among different groups was calculated by one way ANOVA with Bonferroni test. Values were measured statistically substantial at p≤ 0.05.