Biochemical Determinations
After 28 days treatment blood was collected directly from heart and mice
were sacrificed for different biochemical analysis and histology. Blood
glucose (at 7th, 14th,
21st and 28th day), lipid profile
(Cholesterol, triglycerides, HDL-C, LDL-C and VLDL-C), LFT (ASAT,
ALAT, ALP, bilirubin and total protein), RFT (Albumin,
creatinine and urea), antioxidant activity (SOD, CAT and LPO) and
hematological parameters were analyzed. Histopathological studies were
also carried out. See (figure 1) for complete research plan.
Arithmetical
analysis
GraphPad prism (version 5.0) was used for arithmetical studies. All
values were expressed as mean±standard error of mean. Statistical
difference among different groups was calculated by one way ANOVA with
Bonferroni test. Values were measured statistically substantial at p≤
0.05.