Sanger sequencing-
In our sequenced samples we identified 15 unique bacterial genera. The
majority of isolates were in the genus Bacillus(~13% of our sequenced isolates) but a sizable amount
belonged to other taxonomic groups including Microvirga(~6 %), Micromonospora (~5 %),Skermanella (~5 %), and Paenibacillus(~3 %). Isolates from four genera were isolated from
our 1950 sample. We identified isolates from 12 genera in our two
nodules taken from the 2004 specimen. We found isolates from three
genera from our nodule taken from a 2015 specimen (Fig. 3).