Figure 1. SSV-Seq 2.0 workflow. The SSV-Seq protocol was
described in Lecomte et al. (Lecomte et al., 2019) (left panel). The
optimized SSV-Seq 2.0 protocol is represented on the right side. A total
quantity of 8x1011 vector genomes of purified rAAV
vector sample is required as input. A pretreatment with a DNases
cocktail can be performed prior to DNA extraction to specifically
identify and quantify DNA that are encapsidated in rAAV capsids. A
second strand synthesis step is carried out, followed by the PCR-free
DNA library preparation. Finally, high-throughput sequencing is
performed using Illumina HiSeq platform (rapid run 2x94 pb).