Quality control of the DNA sequencing libraries
The quality of the DNA libraries was controlled by electrophoresis on a
microchip using the High Sensitivity DNA kit (Agilent Technologies,
Santa Clara, CA). Electrophoregrams were obtained after migration in the
Agilent Bioanalyzer 2100 instrument and after analysis using the Agilent
2100 Expert Software. The total DNA concentration of the libraries was
determined on the Qubit 4 fluorometer using the Qubit 1X dsDNA High
Sensitivity Assay kit (ThermoFisher Scientific, Waltham, MA).
Adapter-ligated DNA fragments were quantified by real-time PCR using the
Universal qPCR KAPA SYBR Fast kit (Roche, Basel, Switzerland) before
Illumina sequencing.