Authors contribution
E.L. designed the study, performed experiments and bioinformatics analyses, compiled data in figures and tables and wrote the manuscript. S.S. performed experiments, optimizations of the PCR-free protocol and interpreted data. M.B. performed experiments and created the SSV-Coverage program. A.G-D developed the NTContent program. V.B. supervised the production of AAV viral vectors and reviewed the manuscript. O.A. provided funding and supervised research. M.P-B and E.A. coordinated the work, supervised research, interpreted data, provide funding and wrote the manuscript.