Figure 4. Sequencing coverage along the rAAV vector genome and the plasmid backbone. Sequencing libraries were prepared from a purified rAAV-CAG-GFP vector batch following the original SSV-Seq protocol (blue shades) or SSV-Seq 2.0 PCR-free protocol (green shades). Each library was prepared in triplicate. The sequencing coverage is normalized by dividing the read coverage at each base by the sum of the coverage for all bases mapped along the vector plasmid. Grey boxes represent the two 300 bp-region where the number of reads drops. The vector plasmid map is represented above the graph.