Table 1. Percentage of DNA species in a rAAV vector batch after high-throughput sequencing: comparison of the original SSV-Seq protocol with the SSV-Seq2.0 PCR-free method. The relative quantity of each DNA species is indicated in percentages. The rAAV8-CAG-GFP stock was produced by plasmid transfection in HEK293 cells and purified by ultracentrifugation on cesium chloride gradients. For SSV-Seq computational analyses, Fastq files were processed by ContaVect. (*) This reference corresponds to the human genome (GRCh38) and the Ad5 genome fragment integrated into the HEK293 cell line genome. Libraries were prepared in triplicates, without DNase pre-treatment (total residual DNA).