Data and Statistical analysis
All data and statistical analysis comply with the recommendations on
experimental design and analysis in pharmacology (Curtis et al, 2018).
All data presented as mean ± standard error of the mean (SEM) unless
otherwise stated. Statistical differences between treatments were
determined by analysis of variance (ANOVA) followed by Tukey’s multiple
comparison post-hoc tests where appropriate. One-way ANOVA were used for
three of more unmatched groups. Two-way ANOVA were used to analyse data
when response was influenced by two categorized factors of interest. For
comparisons between two groups of results, regular unpaired t-tests were
used to generate the P value. All statistical analyses were performed
using GraphPad PrismTM for Microsoft Windows®
(Versions 8, Graphpad software®, USA)
where p <0.05 was
accepted as significant for all cases.