Ebselen prevents CS-induced endothelial dysfunction
As chronic CS exposure caused significant lung inflammation as well as
heightened lung and vascular oxidative stress, we sought to investigate
the effect of antioxidant treatment on vascular endothelial function.
Sham + Veh-treated mice showed an ~90%
Rmax, while CS + Veh-treated mice showed an
~40% Rmax to ACh (Figure 4A),
confirming CS-induced endothelial dysfunction as also demonstrated in
Figure 1. CS-exposed mice treated with ebselen were completely protected
from the CS-induced endothelial dysfunction evidenced by the maximal
relaxation of ~90% to ACh. In addition, ebselen did not
affect vascular endothelial function in sham-exposed mice (Figure 4A),
suggesting its protective effects are specific to CS exposure. Like the
data in Figure 1, smooth muscle relaxant responses to SNP were unaltered
irrespective of CS exposure or ebselen treatment (Figure 4B).