Materials
The Suppliers of the following compounds are as follows: Winfield red
cigarettes (Phillip Morris, Australia); ebselen (Sapphire Bioscience,
Australia); Carboxymethyl Cellulose (Sigma-Aldrich, USA); sodium
pentabarbitone (Virbac, Australia); acridine orange/ethidium bromide
(Invitrogen, USA); Kwik-Diff® reagent 1, fixative
(Thermo Fisher Scientific, USA); Hemacolour® rapid
staining of blood smear (eosin solution) (Merck, USA);
Hemacolour® rapid staining of blood smear (thiazine
solution) (Merck, USA); RNeasy Mini Kit (Qiagen, Germany); High Capacity
RNA-to-cDNA kit (Thermo Fisher Scientific, USA); pre-developed TaqMan
primers (Thermo Fisher Scientific, USA), CRP ELISA Kit (RayBiotech,
USA); U46619 (Cayman Chemical, USA); acetylcholine (Thermo Fisher, USA);
sodium nitroprusside (Thermo Fisher, USA); NOS3/eNOS RRID: AB_2533121
(Thermo Fisher Scientific, USA), 3-NT RRID: AB110282 (Abcam, USA); Goat
anti-mouse IgG (H+L) secondary antibody, Alexa Flour Plus 488 RRID:
AB_2633275 (Thermo Fisher Scientific, USA);
Fluoromount-GTM, with DAPI (Thermo Fisher Scientific,
USA) .