Quantification of K18:
Samples were stored at -80°C before analysis. K18 was quantified in
samples from healthy volunteers and patients in the ALISTER study, with
these samples stored for a maximum of just over 2 years (113 weeks)
(median [IQR]: 48.4 [28.7-77.8] weeks) prior to analysis. K18
was quantified in serum samples from the SAEFRIF trial, these samples
were stored for a maximum of 1 year (median [IQR]: 19.1
[11.1-26.2] weeks). Total K18 was quantified using the Peviva M65
classic ELISA (Bioaxxes, Tewkesbury, UK), according to the
manufacturer’s instructions. Samples were measured in duplicate. CV
values for the dataset were (median [IQR]: 2.85%,
[1.33-4.97%]). Reproducibility was determined using the provided
plate controls.