Figure 1. The clinical scores, macrophage percentages in spleen, pathology and cytokines changes in EAE. (a) EAE group developed clinical signs on day 10 (onset) p.i. On day 22 p.i., the symptoms of EAE peaked, then the signs began to ease gradually on day 25 (recovery). The control group had no symptoms. At the same time, the weight of EAE group gradually decreased until the lowest point on day 22 p.i., and then increased, which was consistent with the changes of symptoms. (b) Macrophage percentages in the spleens were measured at different stage of EAE: Macrophages in the spleen increased at the onset, then will gradually decreased at peakhour and recover stage; M1 macrophages increased at the onset, and decreased at the peak hour and recover; M2 macrophages increased at the peak hour, then decrease at recover. (c) Inflammatory cell infiltration around the blood vessels (HE stains) and demyelination in the spinal cord (LFB stains are more severe in the EAE mice than in the naïve mice (normal mice). (d) All the pro-inflammatory cytokines increased at the disease onset and IL-17A, IL-6, TNF-α, IL-12p70 decrease at the peak hour. While anti-inflammatory cytokines, IL-4 and IL-10, significantly increased during the peak period.