Histological analysis
The spinal cord was removed from the spinal canal and embedded in
paraffin. Multiple horizontal sections(5-6 µm thick) of the spinal cord
were stained with hematoxylin-eosin(HE) and replicate sections were
stained with luxol fast blue(LFB) violet for evaluation of the extent of
inflammatory cell infiltration and demyelination. The number of
infiltrated inflammatory cells and the degree of demyelination in each
mouse were averaged from 10 complete cross-sections of the spinal cord.