Cell Culture
Primary KCs were prepared from the livers of 8- to 9-week-old male
C57BL/6 mice and cultured in 10% FBS RPMI 1640 medium .A single-cell
suspension form livers was prepared as described
previously[53].
BMDMs were isolated from femoral and tibia bone marrow and then cultured
in RPMI1640 medium(10%FBS) with 100 ng/mL M-CSF (Peprotech) for 5
days to fully differentiate macrophages. Primary mouse hepatocytes were
obtained from the livers of male C57BL/6J mice (8 weeks of age) and
cultured as previously
reported[54]. HepG2
cells, a human hepatic cell line, were purchased from ATCC (Manassas,
VA, USA, RRID: CVCL 0027) and cultured in DMEM medium containing 10%
fetal bovine serum (FBS), 50 mg/mL penicillin/streptomycin and 2 mmol/L
glutamine. Cells (~90% confluence) received treatment
in serum-free medium.