3.3 GO and KEGG enrichment
The GO was used to identify key biological functions corresponding to 1
of 3 term categories, namely, biological process, cellular component,
and molecular function. Results showed that the most significant GO
terms in each term category for 173 significant DEGs were ”antigen
processing and presentation of peptide or polysaccharide antigen via MHC
class II”, ”MHC class II protein complex”, and ”MHC class II receptor
activity” (Table 2, Fig. 2A). Furthermore, the identified significantly
enriched KEGG pathways of significant DEGs were demonstrated to be about
infection, inflammation, and immune response (Table 2, Fig. 2B). Among
them, virus infection, antigen processing and presentation, and B cell
receptor were identified as those that might play an important role in
the pathogenesis of BPD.
Similarly, the results of GSE32472 were analyzed by Pietrzyk et al.
suggested that the signaling pathway of the hematopoietic cell lineage,
allograft rejection, asthma, intestinal immune network for IgA
production, and cell adhesion
molecules (CAMs) were also involved in BPD (Pietrzyk et al., 2013).
Moreover, the hematopoietic cell lineage signaling pathway could be
obtained in all 3 measurements in their study. Regarding the results of
GSE125873 which were analyzed by Ryan et al., they also found the
signaling pathway of CAMs, phagosome, systemic lupus erythematosus, and
leishmaniasis. In addition, they also obtained the processes of ”antigen
processing and presentation of exogenous peptide antigen via MHC class
II”, ”antigen processing and presentation of peptide or polysaccharide
antigen via MHC class II” and ”MHC class II receptor activity” in the
terms of the GO-BP category (Ryan et al., 2019).