2.2 Identification of differentially expressed miRNAs and
differentially expressed genes
We compared samples from infants with BPD with those from normal preterm
infants in GSE108755 and GSE108754 to identify DEMs and DEGs. The
comparison was conducted using a Limma R package based on NetworkAnalyst
3.0
(https://www.networkanalyst.ca/NetworkAnalyst/home.xhtml)
(Zhou et al., 2019). The ”P value < 0.05” and ”|log2
fold change (FC)| ≥
1”
indexes were set as filtering criteria to screen for significant DEMs.
False-positive results could be corrected using the adjusted P value
from the Benjamini-Hochberg method, and the “adjusted P value
< 0.05” and “|log2 fold change (FC)|≥1”
indexes were used as primary filtering criteria to screen for DEGs.