2.6 Construction of a miRNA-mRNA regulatory network
IntAct (http://www.ebi.ac.uk/intact/),21 and MIPS (http://mips.helmholtz-muenchen.de/proj/ppi/)22were used to collect empirically validated mouse protein-protein interaction (PPI) data, before they were merged into an integrated empirically validated PPI dataset. Meanwhile, TarBase (http://carolina.imis.athena-innovation.gr/diana_tools/web/index.php?r=tarbasev8/index)23,24 was applied to collect empirically validated mouse miRNA-mRNA pair data. Thus, these verified data, together with our predicted candidate miRNA-mRNA pairs data from Section 2.5, were integrated into a global microRNA-mRNA regulatory network.25,26 The visual representation of the network was drawn using Cytoscape.27 The analysis pipeline for the complete analysis strategy was shown in Figure 1A.