3.1 Differentially expressed miRNAs and mRNAs
As we previously described, the BPD mouse model was established
successfully after 21 days of hyperoxia exposure (Figure 1B). To
validate microarray data, we randomly select 6 miRNAs1or 6 mRNAs to detect their expression levels by qRT-PCR (E-image 1),
results revealed that these two methodologies had high internal
consistency, and we can use these microarray data for further
analysis.1,28 According to the analysis pipeline
(Figure 1A), we first analyzed differentially expressed miRNAs and
mRNAs. Using a 1.5-fold difference in expression as a cutoff, a total of
192 differentially expressed miRNAs (74 downregulated and 118
upregulated) and 1225 differentially expressed mRNAs (479 downregulated
and 746 upregulated) were identified between BPD mice and
normoxia-control mice (E-Table 1 and E-Table 2, respectively). The top
ten miRNAs and mRNAs were shown in E-Table 3 and E-Table 4,
respectively.