2.8 Quantitative reverse transcription-PCR (qRT-PCR) validation
and statistical analysis
Reverse transcription (RT) reactions and qRT-PCR were carried out as
previously described. All miRNAs were normalized to u6, and all mRNAs
were normalized to GAPDH. The relative expression levels of each gene
were calculated using the 2−ΔΔCt method as previously
described.1,28 All data were expressed as the mean±
standard deviation. Student’s t-test was used for comparisons between
two groups. The statistical significance level was set at p <
0.05.
RESULTS