FIGURE LEGENDS
FIGURE 1. Analysis pipeline (A) for hyperoxia-exposed neonatal
mice model (B). (B) showed lung structure was markedly abnormal, with
enlarged air spaces and simpliļ¬ed structure, in neonatal mice exposed to
hyperoxia from P7 to P21, compared with air controls. Bars represent m.
FIGURE 2. Enriched GO terms analysis and KEGG pathway analysis
for differentially expressed mRNAs. (A) Top 10 significantly enriched
GO terms of downregulated mRNA-BP. (B) Top 7 significantly pathways of
downregulated mRNA. (C) Top 10 significantly enriched GO terms of
upregulated mRNA-BP. (D) Top 9 significantly pathways of downregulated
mRNA.
FIGURE 3. Enriched GO terms analysis for predicted targets of
each differentially expressed miRNA. (A) Left: the enriched GO BP of
let target genes; Right: the visualization of let regulated targets. (B)
Left: the enriched GO BP of miR-21 target genes; Right: the
visualization of miR-21 regulated targets. (C) Left: the enriched GO BP
of miR-205 target genes; Right: the visualization of miR-205 regulated
targets. (a-c) The depth of color represented the value of (FC-1) if FC
larger than 1, otherwise represented the value of (-1/FC+1). Red meant
upregulation, green meant downregulation while white meant no change.
FIGURE 4. Immune and inflammation-related miRNA-mRNA regulatory
network. (A) Visualization of the global miRNA-mRNA regulated network.
Red node represented gene, green node represented miRNA. Grey edge
represented predicted miRNA-mRNA pairs, yellow edge represented
empirically validated miRNA-mRNA pairs while blue edge represented PPI.
(B) The visualization of the first immune related subnetwork. (C) The
visualization of the second immune related subnetwork. (b-c) The
rectangle represented miRNA while circle represented gene. The depth of
color represented the value of (FC-1) if FC larger than 1, otherwise
represented the value of (-1/FC+1). Red meant upregulation, green meant
downregulation while white meant no change. Grey node represent the gene
whose expression can not be detected in our profiles. Red edge
represented the empirically validated miRNA-mRNA pairs, grey edge
represented our predicted miRNA-mRNA pairs while blue edge represented
PPI.
E-image 1. Correlation of 6 mRNAs levels between mRNA microarray
and real-time PCR analyses. (A) The expression levels were
independently assessed by microarray and real-time PCR, and (B) the
results in both methodologies demonstrated very high internal
consistency with a correlation coefficient (R2) of 0.904, and p value
< 0.01 (Pearson).