FIGURE LEGENDS
FIGURE 1. Analysis pipeline (A) for hyperoxia-exposed neonatal mice model (B). (B) showed lung structure was markedly abnormal, with enlarged air spaces and simplified structure, in neonatal mice exposed to hyperoxia from P7 to P21, compared with air controls. Bars represent m.
FIGURE 2. Enriched GO terms analysis and KEGG pathway analysis for differentially expressed mRNAs. (A) Top 10 significantly enriched GO terms of downregulated mRNA-BP. (B) Top 7 significantly pathways of downregulated mRNA. (C) Top 10 significantly enriched GO terms of upregulated mRNA-BP. (D) Top 9 significantly pathways of downregulated mRNA.
FIGURE 3. Enriched GO terms analysis for predicted targets of each differentially expressed miRNA. (A) Left: the enriched GO BP of let target genes; Right: the visualization of let regulated targets. (B) Left: the enriched GO BP of miR-21 target genes; Right: the visualization of miR-21 regulated targets. (C) Left: the enriched GO BP of miR-205 target genes; Right: the visualization of miR-205 regulated targets. (a-c) The depth of color represented the value of (FC-1) if FC larger than 1, otherwise represented the value of (-1/FC+1). Red meant upregulation, green meant downregulation while white meant no change.
FIGURE 4. Immune and inflammation-related miRNA-mRNA regulatory network. (A) Visualization of the global miRNA-mRNA regulated network. Red node represented gene, green node represented miRNA. Grey edge represented predicted miRNA-mRNA pairs, yellow edge represented empirically validated miRNA-mRNA pairs while blue edge represented PPI. (B) The visualization of the first immune related subnetwork. (C) The visualization of the second immune related subnetwork. (b-c) The rectangle represented miRNA while circle represented gene. The depth of color represented the value of (FC-1) if FC larger than 1, otherwise represented the value of (-1/FC+1). Red meant upregulation, green meant downregulation while white meant no change. Grey node represent the gene whose expression can not be detected in our profiles. Red edge represented the empirically validated miRNA-mRNA pairs, grey edge represented our predicted miRNA-mRNA pairs while blue edge represented PPI.
E-image 1. Correlation of 6 mRNAs levels between mRNA microarray and real-time PCR analyses. (A) The expression levels were independently assessed by microarray and real-time PCR, and (B) the results in both methodologies demonstrated very high internal consistency with a correlation coefficient (R2) of 0.904, and p value < 0.01 (Pearson).