2.8 Quantitative reverse transcription-PCR (qRT-PCR) validation and statistical analysis
Reverse transcription (RT) reactions and qRT-PCR were carried out as previously described. All miRNAs were normalized to u6, and all mRNAs were normalized to GAPDH. The relative expression levels of each gene were calculated using the 2−ΔΔCt method as previously described.1,28 All data were expressed as the mean± standard deviation. Student’s t-test was used for comparisons between two groups. The statistical significance level was set at p < 0.05.
RESULTS