3.1 Differentially expressed miRNAs and mRNAs
As we previously described, the BPD mouse model was established successfully after 21 days of hyperoxia exposure (Figure 1B). To validate microarray data, we randomly select 6 miRNAs1or 6 mRNAs to detect their expression levels by qRT-PCR (E-image 1), results revealed that these two methodologies had high internal consistency, and we can use these microarray data for further analysis.1,28 According to the analysis pipeline (Figure 1A), we first analyzed differentially expressed miRNAs and mRNAs. Using a 1.5-fold difference in expression as a cutoff, a total of 192 differentially expressed miRNAs (74 downregulated and 118 upregulated) and 1225 differentially expressed mRNAs (479 downregulated and 746 upregulated) were identified between BPD mice and normoxia-control mice (E-Table 1 and E-Table 2, respectively). The top ten miRNAs and mRNAs were shown in E-Table 3 and E-Table 4, respectively.