3.2 GO and KEGG pathway enrichment of differentially expressed mRNAs
In total, 479 downregulated and 746 up-regulated mRNAs were analyzed with GO and KEGG pathway enrichment analysis. For downregulated genes, there were 35 GO enrichment processes with FDR<0.01. The top ten enriched BP were mainly related to immune and inflammation processes, such as, positive regulation of natural killer cell mediated cytotoxicity, and response to lipopolysaccharide (Figure 2A, E-Table 5). Furthermore, there were 7 enriched KEGG pathways with FDR<0.01, and these pathways were also largely related to immune and inflammation processes: cytokine-cytokine receptor interaction, natural killer cell mediated cytotoxicity, and Toll-like receptor signaling pathway (Figure 2B, E-Table 6). For the 746 upregulated genes, there were 62 GO enrichment processes with FDR<0.01. The top ten enriched BP were primarily related to heart muscle signal transduction, gluconeogenesis, and glycolysis processes (Figure 2C, E-Table 7). There were 9 enriched KEGG pathways with FDR<0.01 and these pathways were mainly related to olfactory transduction, ECM-receptor interaction, PPAR signaling pathway, cell Communication, gluconeogenesis and glycolysis pathways (Figure 2D, E-Table 8).