2.6 Construction of a miRNA-mRNA regulatory network
IntAct (http://www.ebi.ac.uk/intact/),21 and
MIPS
(http://mips.helmholtz-muenchen.de/proj/ppi/)22were used to collect empirically validated mouse protein-protein
interaction (PPI) data, before they were merged into an integrated
empirically validated PPI dataset. Meanwhile, TarBase
(http://carolina.imis.athena-innovation.gr/diana_tools/web/index.php?r=tarbasev8/index)23,24 was applied to collect empirically validated
mouse miRNA-mRNA pair data. Thus, these verified data, together with our
predicted candidate miRNA-mRNA pairs data from Section 2.5, were
integrated into a global microRNA-mRNA regulatory
network.25,26 The visual representation of the network
was drawn using Cytoscape.27 The analysis pipeline for
the complete analysis strategy was shown in Figure 1A.