Cell sorting and Culture
Fluorescence-activated cell sorting (FACS) were used to purify
CD27+IgD+ B cells, 10ml blood was
collected from SLE patients and healthy controls, peripheral blood
mononuclear cells (PBMCs) were isolated from fresh blood samples using
Ficoll density-gradient centrifugation, and then were stained as
described above. After that, the aimed cells were harvested into the
collection solution (RPMI 1640 supplemented with 10% FBS and 2%
antibiotics) using FACS Aria II flow cytometry sorter according to the
manufacturer’s instructions. The purified cells were further analyzed
after sorting, the purity of which was 95–99%.