Flow cytometry analysis
For CD27+IgD+ B cell detection, 2ml
blood was collected from SLE patients and healthy controls, and 100µl
fresh whole blood cells was extracted and stained with the following
antibodies:APC-CY7-conjugated anti-CD19 (Biolegend, San Diego, CA,
USA), APC-conjugated anti-CD27 (eBioscience, San Diego, CA, USA), and
FITC-conjugated anti-IgD (eBioscience). Then the red blood cells were
lysed using the RBC lysis buffer (MultiSciences, Hangzhou, China) and
the left cells were analyzed on FACS
Aria II. Dead cell exclusion was performed by scatter profiles and 7-AAD
staining during all the flow cytometric analyses.