Statistics analysis
All statistical calculations were performed using the statistical
software program SPSS 17.0 (SPSS, Chicago, IL, USA). Differences between
various groups were evaluated by the Student’s t-test, paired t-test,
one-way ANOVA test, or spearman test, and were statistically significant
at P < 0.05.ResultsThe frequencies ofCD27+IgD+B cells are significantly decreased in SLE patients
B cells could be divided into four subsets based on their expression of
CD27 and IgD, including naïve B cells
(CD27-IgD+), Innate-like B cells
(CD27+IgD+), switched memory B cells
(CD27+IgD-), and double negative B
cells (CD27-IgD-) as demonstrated in
Figure 1A. To reveal the role of
CD27+IgD+B cells in SLE
pathogenesis, we first compared their frequencies between SLE patients
and healthy individuals. As shown in Figure 1B and C, compared to the 50
healthy volunteers, the frequencies of
CD27+IgD+B cells were significantly
decreased in the peripheral blood of 50 SLE patients.