ELISPOT
ELISPOT was carried out with ELISpotPLUS Human IgM Kit (MABTECH AB,
Sweden). In brief, 104 purified
CD27+IgD+ B cells and other B cell
types from SLE and HC patients, respectively, were assessed for IgM
after incubation with anti-CD40 (3µg/ml, eBioscience) and CpG (10µg/ml,
Invivogen, USA) for 24h. An ImmunoSpot Analyzer (Cellular Technology
Ltd., USA) was utilized for data analysis.