Figure 3 IgM-producing abilities of CD27+IgD+B cells in SLE and healthy control patients. Flow cytometry-purified CD27+IgD+B cells from four healthy donors and four active SLE cases (SLEDAI>5) were assessed by ELISPOT (A) and real-time PCR (B) for IgM detection. Representative charts and quantitation are shown (t-test, *P<0.05, ***P<0.001).