Data analysis
The slope factor (K) and the midpoint (V1/2) for
activation and inactivation were calculated using standard Boltzmann
functions: 1/(1 + exp ((V1/2activation –
V)/kactivation)) for activation and (1 - C)/(1 + exp ((V
– V1/2inactivation)/kinactivation)) +
C) for inactivation. V is the voltage. The window current was
obtained using equation 1: (1/(1 + exp ((V1/2activation– V)/kactivation)) x ((1 - C)/(1 + exp ((V –
V1/2inactivation)/kinactivation)) + C) x
100, which is the probability of having the channel in the open state.