Analysis of tocopherols
A Shimadzu Prominence-I LC-2030A high performance liquid chromatograph
(HPLC), equipped with a photodiode array detector (scanning 200-500 nm,
set wavelength for data collection and quantitation at 295 nm) and
fluorescence detector (excitation 292 nm, emission at 344 nm) in series
was used to analyze tocopherols in oils as described by (Winkler-Moser
et al., 2023). Tocopherols were identified by retention time of
commercial standards, and peak confirmation with a photodiode array
detector scanning between 200-500 nm. Quantitation was conducted with
external standard curves using the fluorescence detector. Analysis was
conducted in duplicate.