2.4. Estimation of exopolysaccharides (EPS)
The content of EPS was determined according to Sharma et al. (2008). The
100 ml culture from each set was centrifuged (3000 g) for 15 min and
cell free supernatant (containing EPS) was concentrated ten folds by
evaporating at 40 °C. The obtained precipitate was washed thrice with
isopropanol and dried at 37 °C. Lastly, hydrolysate was analyzed for
glucose (Seifter et al., 1959) and EPS content was calculated with the
help of a standard curve prepared with the help of glucose.