2.9.2. Non-enzymatic antioxidants
The content of cysteine (Cys) in each sample was determined by recording
the absorbance of reaction mixture at 560 nm following the method of
Gaitonde (1967), and the amount of formed Cys was calculated by the help
of standard curve.
Similarly, the content of proline (Pro) in each sample was determined by
recording the absorbance of toluene layer of the reaction mixture at 520
nm following the method of Bates et al. (1973), and the amount of formed
Pro was calculated by the help of standard curve.
The content of non-protein thiols (NPTs) in each sample was determined
by recording the absorbance of reaction mixture at 412 nm following the
method of Ellman et al. (1959) and the amount of formed NPTs was
calculated by the help of standard curve.