2.4. Estimation of exopolysaccharides (EPS)
The content of EPS was determined according to Sharma et al. (2008). The 100 ml culture from each set was centrifuged (3000 g) for 15 min and cell free supernatant (containing EPS) was concentrated ten folds by evaporating at 40 °C. The obtained precipitate was washed thrice with isopropanol and dried at 37 °C. Lastly, hydrolysate was analyzed for glucose (Seifter et al., 1959) and EPS content was calculated with the help of a standard curve prepared with the help of glucose.