2.8.2. Histochemical staining (in-vivo visualization)
For the quantification of O2•─ and
H2O2 production in cyanobacterial cells,
the in-vivo staining with the help of nitrobluetetrazolium (NBT)
and 3, 3 diaminobenzidine (DAB)-HCl, respectively was achieved following
the method of Forster et al. (2005). Similarly, for the visualization of
MDA equivalents and membrane integrity, cyanobacterial cells were
stained with Schiff’s reagent and Evan’s blue following the method of
Pompella et al. (1987) and Yamamoto et al. (2001), respectively and the
photographs were captured with the help of microscope (Leica, model-DM
2500).