2.7.2.2. Glutamine synthetase (GS; EC 6.3.1.2) activity
Glutamine synthetase activity was determined by the formation ofγ -glutamylhydroxamate (transferase assay) following the method of
Mérida et al. (1991). One unit (U) GS activity is defined as 1 nmolγ -glutamylhydroxamate formed min–1.
2.7.2.3. Glutamate synthase (Fd-GOGAT; EC1.4.1.13)
activity
Glutamine 2-oxoglutarate aminotransferase (Fd-GOGAT) activity was
estimated by following the method of Navarro et al. (1995). One unit of
GOGAT activity is defined as 1 nmol glutamate formed
min−1.