2.8.1. Biochemical analysis
The content of superoxide radicals (SOR;
O2•─) in all the treatments was
determined by recording the absorbance of colored aqueous solution at
530 nm following the method of Elstner and Heupel (1976), and the amount
of formed O2•─ was quantified using
the standard curve prepared with the help of NaNO2.
Likewise, the content of hydrogen peroxide
(H2O2) in each sample was determined by
recording the absorbance of cleared solution at 390 nm following the
method of Velikova et al. (2000), and the amount of formed
H2O2 was quantified using the standard
curve prepared with the help of H2O2.
Lipid peroxidation in each sample was quantified in terms of
malondialdehyde (MDA) equivalents by recording the absorbance at 532 and
600 nm following the method of Heath and Packer (1968) and the content
of MDA equivalents was quantified using an extinction coefficient 155
mM−1 cm−1.