2.9.2. Non-enzymatic antioxidants
The content of cysteine (Cys) in each sample was determined by recording the absorbance of reaction mixture at 560 nm following the method of Gaitonde (1967), and the amount of formed Cys was calculated by the help of standard curve.
Similarly, the content of proline (Pro) in each sample was determined by recording the absorbance of toluene layer of the reaction mixture at 520 nm following the method of Bates et al. (1973), and the amount of formed Pro was calculated by the help of standard curve.
The content of non-protein thiols (NPTs) in each sample was determined by recording the absorbance of reaction mixture at 412 nm following the method of Ellman et al. (1959) and the amount of formed NPTs was calculated by the help of standard curve.