2.8.2. Histochemical staining (in-vivo visualization)
For the quantification of O2•─ and H2O2 production in cyanobacterial cells, the in-vivo staining with the help of nitrobluetetrazolium (NBT) and 3, 3 diaminobenzidine (DAB)-HCl, respectively was achieved following the method of Forster et al. (2005). Similarly, for the visualization of MDA equivalents and membrane integrity, cyanobacterial cells were stained with Schiff’s reagent and Evan’s blue following the method of Pompella et al. (1987) and Yamamoto et al. (2001), respectively and the photographs were captured with the help of microscope (Leica, model-DM 2500).