2.1. Chemicals, media and solutions
All chemical and biochemical materials were in analytical grade, and
buffer solutions were prepared using Milli-Q water (Millipore, USA).
Then, bovine serum albumin (≥99.5%, BSA, 66.4 kDa) and Blue dextran
2000 (2000 kDa) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
In addition, potassium chloride was obtained from Mojallali Co. (Tehran,
Iran). Further, Tris (hydroxyl methyl) amino methane was purchased from
Sinchem (Jiangsu, China). In the next procedure, SEC and IEX were
conducted on chromatographic media Sup-200 and Q-Sepharose XL virus
license, respectively. Finally, chromatography columns XK 16 and XK 26
and size exclusion/ ion-exchange media were purchased from GE Healthcare
(Illinois, USA).