2.1. Chemicals, media and solutions
All chemical and biochemical materials were in analytical grade, and buffer solutions were prepared using Milli-Q water (Millipore, USA). Then, bovine serum albumin (≥99.5%, BSA, 66.4 kDa) and Blue dextran 2000 (2000 kDa) were purchased from Sigma-Aldrich (St. Louis, MO, USA). In addition, potassium chloride was obtained from Mojallali Co. (Tehran, Iran). Further, Tris (hydroxyl methyl) amino methane was purchased from Sinchem (Jiangsu, China). In the next procedure, SEC and IEX were conducted on chromatographic media Sup-200 and Q-Sepharose XL virus license, respectively. Finally, chromatography columns XK 16 and XK 26 and size exclusion/ ion-exchange media were purchased from GE Healthcare (Illinois, USA).