3. Results and Discussions
AEC with strong anion exchanger immobilized on the surface of the Sepharose and high sample loading capability is considered as a good candidate for acidic environmentally labile virus particles like FMDV, which can accomplish the most important criteria such as speed and capacity for capturing step. It can remove low FMDV loading limitation in the first column in 2D-SEC×SEC method (Bagheri et al., 2018). High resolution SEC fractionation media, in the second column, like Sup-200 as a final polishing step in combination with AEC in two-dimension configuration 2D-AEC×SEC should provide desirable resolution and selectivity for active ingredient of FMD vaccine and critical impurities, rDNA and NSPs. Fig.1 represents a snapshot of the whole developed purification procedure. During the method development, mathematical modeling and simulation are applied in the first dimension for evaluating the reliability of elution pattern of FMDV particles, rDNA and bovine serum albumin (BSA) as a marker of NSPs, as well as in the second dimension based on the pervious study for providing more evidence in producing NSPs free vaccine products (Bagheri et al., 2018).