2.8. SDS-PAGE analysis
Purified fractions obtained from 2D-AEC×SEC were analyzed by 15% sodium
dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) under
the reduced condition. Briefly, virion particles were directly
resuspended in SDS loading buffer, boiled at 100 0C
for 10 minutes, and incubated at 55 0C for 2 h. The
SDS-PAGE were set as 5% acrylamide/N,N bis-acrylamide for the stacking
gel and 15% for the resolving gel, and a high range of pre-stained
protein standards from Sinaclon Co. (Tehran, Iran) (MW 25–180 kDa) was
used as the molecular weight marker. The gels were stained with
Coomassie Brilliant Blue G-250 after electrophoresis to visualize the
protein profiles on the polyacrylamide gel.