2.8. SDS-PAGE analysis
Purified fractions obtained from 2D-AEC×SEC were analyzed by 15% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) under the reduced condition. Briefly, virion particles were directly resuspended in SDS loading buffer, boiled at 100 0C for 10 minutes, and incubated at 55 0C for 2 h. The SDS-PAGE were set as 5% acrylamide/N,N bis-acrylamide for the stacking gel and 15% for the resolving gel, and a high range of pre-stained protein standards from Sinaclon Co. (Tehran, Iran) (MW 25–180 kDa) was used as the molecular weight marker. The gels were stained with Coomassie Brilliant Blue G-250 after electrophoresis to visualize the protein profiles on the polyacrylamide gel.