3.6. Performance of developed 2D-AEC×SEC for purification FMDV
Performance of the constructed 2D-AEC×SEC approach was summarized in Table 1 . In capturing step on Q-Sepharose virus licensed as first dimension about 89.8 recovery and 150-fold of purification were achieved. In addition, further purification led to 18-fold of purification with 92.20% recovery on Sup-200 as the second dimension in the final polishing step. Further, the whole procedure was performed with 77.91 % recovery in the proposed scheme for the production of NSP free FMDV vaccine. According to the HP-SEC data, the purified pooled virus fraction has purity higher than 85.00 % in the polishing step. Interestingly, rDNA in the primary feed stock decreased from 81.30 ng to 8.01 ng. Thus, over 90.10% of host cell rDNA was removed from the final product.