3. Results and Discussions
AEC with strong anion exchanger immobilized on the surface of the
Sepharose and high sample loading capability is considered as a good
candidate for acidic environmentally labile virus particles like FMDV,
which can accomplish the most important criteria such as speed and
capacity for capturing step. It can remove low FMDV loading limitation
in the first column in 2D-SEC×SEC method (Bagheri et al., 2018). High
resolution SEC fractionation media, in the second column, like Sup-200
as a final polishing step in combination with AEC in two-dimension
configuration 2D-AEC×SEC should provide desirable resolution and
selectivity for active ingredient of FMD vaccine and critical
impurities, rDNA and NSPs. Fig.1 represents a snapshot of the
whole developed purification procedure. During the method development,
mathematical modeling and simulation are applied in the first dimension
for evaluating the reliability of elution pattern of FMDV particles,
rDNA and bovine serum albumin (BSA) as a marker of NSPs, as well as in
the second dimension based on the pervious study for providing more
evidence in producing NSPs free vaccine products
(Bagheri et al., 2018).