2.4. Inactivated virus
FMDV strain O2016 was propagated in BHK-21 cell
suspension cultures at industrial scale bioreactor, and finally
inactivated by BEI. Then, cell debris were primarily removed by
centrifuge at 4500 rpm in 20-min filtration through a 0.45 µm membrane
from Sartorius Stedim Biotech (Gottingen,
Germany).
Finally, the crude virus bulk was concentrated about 23-fold using a
100-kDa cut-off diafiltration membrane in a tangential flow filter
cassette from Sartorius Stedim Biotech (Gottingen,
Germany).
The FMDV concentrate samples were originally obtained from Vaccine and
Serum Research Razi Institute (Karaj, Iran) after the
aforementioned cultivation and pretreatment procedure.