2.9 Statistical analysis
Data are reported as box and whisker plots with median, minimum to
maximum, and 10 to 90 percentiles. Figure 4E is plotted as mean ± S.E.M.
The statistical significance between two groups was determined by a
two-tailed unpaired Student’s t test (and among more than two groups it
was determined by repeated measure one-way analysis of variance followed
by Tukey’s post-hoc test) using GraphPad Prism®Version 4.0 software (GraphPad Software Inc, La Jolla, CA, USA).
Difference between two groups was considered significant when
probability values of P were less or equal to 0.05.