2.9 Statistical analysis
Data are reported as box and whisker plots with median, minimum to maximum, and 10 to 90 percentiles. Figure 4E is plotted as mean ± S.E.M. The statistical significance between two groups was determined by a two-tailed unpaired Student’s t test (and among more than two groups it was determined by repeated measure one-way analysis of variance followed by Tukey’s post-hoc test) using GraphPad Prism®Version 4.0 software (GraphPad Software Inc, La Jolla, CA, USA). Difference between two groups was considered significant when probability values of P were less or equal to 0.05.