2.7.1. MTT assay
MTT assay was used to study the proliferation potential and survival of
adipose-derived mesenchymal stem cells (ADMSCs) on different substrates.
After plasma processing, scaffolds were cut into disks (11 mm diameter)
and sterilized with 70% ethanol and ultraviolet light exposure (40
minutes and 120000 µ J/CM2). After being placed in the culture medium
overnight, the cells were seeded on the samples at a density of 5 ×
103 cells/sample, and then incubated in 5%
CO2 at 37 °C in high glucose Dulbecco’s modified
Eagle’s medium, DMEM, supplemented with 10% fetal
bovine serum, FBS, and 1% penicillin-streptomycin. The cytotoxicity
value of the prepared scaffold (PCL and G3-SPION-PCL ) was evaluated
using MTT assay on days 1, 3, 7, and 14 after the seeding of ADMSCs
cells and compared to the tissue culture plates (TCP), as control. The
samples were washed with PBS and incubated in 10% V/V MTT in a DMEM
solution at 37 °C for three h. (Esmaeili,
Soleimani, et al., 2019) After the formation of the formazan crystals
inside the cells seeded on the scaffolds, Then, the supernatant was
removed, DMSO was added to dissolve the Formosan crystals, and the
absorbance of the purple solution was measured at 570 nm in a microplate
reader (BioTek, ELX800, USA).