2.7.5. Cell differentiation
ADMSCs differentiation cultured on the magnetic scaffold to osteoblast
cells was studied under the osteogenic medium, OM, 250 nM dexamethasone,
ten mM beta-glycerol phosphate and 50 μg/ml ascorbic acid-2-phosphate,
DMEM, 10% FBS, 1% penicillin/streptomycin and low frequency pulsed
electromagnetic fields, PEMF, 30 mT ,and 75 Hz, eight h/day.
The osteogenic differentiation of ADMSCs was assessed in different
conditions in 5 groups, the cells seeded on TCP, PCL, and G3-SPION-PCL
under OM, and the cells seeded on G3-SPION-PCL under PEMF, with and
without OM. The cells were under PEMF for 14 days ,and 8 hours per day
and OM was changed every other day for 14 days.