2.7.7. Alkaline phosphatase activity assay
ALP activity, as an early osteogenic marker, is used on days 7 and 14 to
evaluate the osteogenic differentiation. The samples were washed with
PBS and were lysed by RIPA buffer to measure the total protein and ALP
activity. Based on the ALP activity kit (Pars Azmoon, Iran), after
addition of the reagent, the optical density of the samples was measured
at 405 nm and normalized against the total protein (BCA kit, Thermo
Scientific).