2.7.2. DAPI staining
In order to study the cell adhesion on the scaffolds on days 1 and 14,
the nucleus staining of the cells using DAPI was implemented. Cells were
fixed with 4% paraformaldehyde and kept at 4˚C for 20 minutes and at
room temperature for 5 minutes. The samples were subsequently washed
with PBS and stained with DAPI solution for 1 minute in a dark place.
Following the washing, three times, by PBS, the samples were imaged
using a fluorescent microscope (Nikon, Tokyo, Japan).