2.5. Immunohistochemistry.
IHC(immunohistochemistry) samples were held at 4˚C in formalin fixator
after surgical surgery treatment. The immunohistochemical method was
Dako REAL EnVision detection system, and the specific steps were stained
according to the instructions of the kit. Primary antibodies used were
monoclonal rabbit anti-human IL-2(Abcam), polyclonal rabbit anti-human
IL-6(Abcam), monoclonal rabbit anti-human IL-10(Abcam), monoclonal
rabbit anti-human TGF-β(CST), polyclonal rabbit anti-human
INF-γ(Bioworlde), monoclonal rabbit anti-human Foxp3(CST), monoclonal
mouse anti-human PD1(Abcam) and monoclonal rabbit anti-human
PD-L1(Abcam).