2.1. Study patients.
The cohort for this study included 140 female patients with CC diagnosed by cervical and cervical biopsyranging from age 32 to 68 years, CIN from age 31 to 63 years, Chronic Cervicitis from age 28 to 58 and HPV+ diagnosed by HPV genotyping detection from age 28 to 52 years(Fig.1-2). Take peripheral blood, separate plasma or serum for ELISA analysis, and peripheral blood mononuclear cell(PBMC) for flow cytometry detection. Other than a single patient, to the best of our knowledge no patients with CC, CIN, Chronic Cervicitis and HPV+ received any treatment with surgery or chemotherapy prior to the collection of specimens. In addition, samples from five participants of CC were follow-up monitoring detected by ELISA with separated plasma or serum until about 180 days after surgical treatment.