2.1. Study patients.
The cohort for this study included 140 female patients with CC diagnosed
by cervical and cervical biopsyranging from age 32 to 68 years, CIN from
age 31 to 63 years, Chronic Cervicitis from age 28 to 58 and HPV+
diagnosed by HPV genotyping detection from age 28 to 52 years(Fig.1-2).
Take peripheral blood, separate plasma or serum for ELISA analysis, and
peripheral blood mononuclear cell(PBMC) for flow cytometry detection.
Other than a single patient, to the best of our knowledge no patients
with CC, CIN, Chronic Cervicitis and HPV+ received any treatment with
surgery or chemotherapy prior to the collection of specimens. In
addition, samples from five participants of CC were follow-up monitoring
detected by ELISA with separated plasma or serum until about 180 days
after surgical treatment.