2.5. Immunohistochemistry.
IHC(immunohistochemistry) samples were held at 4˚C in formalin fixator after surgical surgery treatment. The immunohistochemical method was Dako REAL EnVision detection system, and the specific steps were stained according to the instructions of the kit. Primary antibodies used were monoclonal rabbit anti-human IL-2(Abcam), polyclonal rabbit anti-human IL-6(Abcam), monoclonal rabbit anti-human IL-10(Abcam), monoclonal rabbit anti-human TGF-β(CST), polyclonal rabbit anti-human INF-γ(Bioworlde), monoclonal rabbit anti-human Foxp3(CST), monoclonal mouse anti-human PD1(Abcam) and monoclonal rabbit anti-human PD-L1(Abcam).