3.3 | Diversity analysis of the rhizosphere
microbial community
A total of 1,079,408 and 1,675,540 high-quality sequences were analyzed
for bacteria and fungi,
respectively. In bacteria, the high,
medium and low carbon sequestration rhizosphere soils contained 1704,
1641, and 1672 OTUs, respectively. A total of 1221 OTU sequences were
common among the three kinds of samples (Figure 1 A). In contrast, 808,
654 and 838 fungal OTUs were identified from the high, medium and low
carbon sequestration rhizosphere soil samples, respectively. Only 254
soil fungal OTU sequences were common among the three sample types
(Figure 1 B). B). The OTUs of fungi in each sample were far fewer than
those of bacteria.
The Chao and Shannon index of bacteria and fungi are shown in Figure 1.
C,D. For bacteria, the Chao index of the different samples fluctuated
between 1476 and 1521, and samples with high carbon sequestration had
slightly higher values that were not
significantly different from those of
the other samples. The Chao index for fungi was approximately 400. The
difference in the Chao index values between fungi and bacteria was
significant (Figure 1C). On the other hand, the Shannon index for
bacteria and fungi from the different families showed only slight
differences, and the differences among samples were not significant
(Figure 1D). These results indicate that overall, combined with the
other parameters used to evaluate the microbial community α-diversity
shown in Table S3, there was little difference in
α-diversity
among the rhizosphere microorganisms in the various samples.