3.2 Correlation between MTHFR C677T/A1298C and clinicopathologic characteristics of pediatric patients with NHL
Among the whole group, 158 patients (42.2%) were genotyped with relation to polymorphisms C677T and A1298C. However, MTHFR A1298C sequencing failed in four patients. No significant differences were found in the clinicopathological features between the two subgroups with or without MTHFR genotyping (TABLE 1). Both the C677T and A1298T alleles exhibited the H-W equilibrium in the studied population (677C > T variant: χ2 = 0.3326, P= 0.5641; 1298A > C variant: χ2 = 0.1010, P = 0.7506). Over half of the patients (83/158; 52.5%) carried the C677T wild-type genotype (CC), while 61/158 (38.6 %) and 14/158 patients (8.9%) carried the heterozygous (CT) and homozygous (TT) genotype, respectively. For the A1298C polymorphism, wild-type (AA), heterozygous (CT) and homozygous (TT) genotypes were observed in 96/154 (62.3 %), 52/154 (33.8 %) and 6/154 patients (3.9 %), respectively. Interestingly, all patients with 677TT (n = 14) exhibited the wild-type 1298AA genotype. TABLE 2 shows the correlation between the C677T/A1298C polymorphisms and the clinicopathologic characteristics of pediatric patients with NHL. C677T showed a correlation with the incidence of all events (P = 0.042). A1298C was found to be significantly associated with NHL subtype (P = 0.045).