Materials and Methods
Forty-five patients diagnosed as having early-stage laryngeal cancer and treated with RT in the otorhinolaryngology department of XXX University Faculty of Medicine between 2002 and 2016 were included in the study. Patients were treated with curative radiotherapy with a linear accelerator with a peak energy of 6 MV. All patients were treated Patients were treated with 225 cGy /28 days in the same radiotherapy center. The demographic and clinical data, pathology reports, prognostic parameters, and survival rates of the patients were retrieved retrospectively from their medical records. Among patients with early-stage patients with recurrent and Cure with the same sampling were studied. Patients with anterior commissure involvement were excluded from the study. The patients were divided into two groups, those who showed complete response with RT (non-recurrent group) and those who developed local recurrence despite RT (recurrent group). The correlation between treatment response and immunohistochemical staining for ALDH1 and CD44 at time of diagnosis was evaluated. Survival rates were also compared between the groups.