Relationships of environmental variables to mucus transcriptomic
profiles
A linear mixed model was used to quantify the percent variance in
transcriptome-wide gene expression that could be explained by water
quality variables. The variables included in the model explained a
median of 68.1% across all genes in the transcriptome-wide count
matrix, leaving a median residual of 31.9% variance unexplained (Figure
7). Conductivity, chlorophyll a, alkalinity, and dissolved oxygen
explained the greatest proportion of variance within the transcriptome
(Figure 3).