of TDF--assumed to approximate the expected therapeutic range--, relative to the replication in the absence of the antiviral. However, this computation is not straightforward as the exact length of the SARS-CoV-2 viral cycle is not known and might substantially vary [21]. Thus, we aimed to estimate a credible range of the TDF inhibition under the assumption that on average, a complete viral cycle remains between 12h and 36h [20] . First we computed the experimental Viral-load Relative ratio (VR) as the ratio between the  2-ΔΔCt  values obtained from each of the TDF-SARS-CoV2 cultures at either 3 or 10 microM divided by the average 2-ΔΔCt  of the control cultures (i.e., without antiviral, n=3). Alternatively, the estimation of VR can be roughly computed by assuming that every increase of 3.3 units in the Ct value corresponds with a 10-fold decrease in the viral load [22].