2.2.3 Total soluble carbohydrate
Total soluble carbohydrate (TSC) was extracted thrice from 100 mg of mature seed using extraction soluble including glacial acetic acid, methanol, and water. It centrifuged at 3000 rpm for 10 min. The mixture of 0.5 mL extraction with 0.5 mL of 5% (v: v) phenol solution and 2.5 mL purred sulfuric acid were heated at 90°C for 30 min (Dubois et al., 1965). The absorbance was read at 490 nm. TSC content was recorded as mg-1dry weight.