2.1. Growth conditions and treatments
This research was organized at the Research Greenhouse of Biology
Department, Shiraz University, Shiraz, Iran from December to February
2018. The Camelina “Soheil cultivar” was selected as plant material
and obtained from Bisetoon Shafa Co., Kermanshah, Iran. Before planting,
the soil samples were canvass with a 2 mm sieve and autoclaved for 3h.
The results of soil analysis are presented in Table 1. According to
these results, essential elements were added to all the pots with 5kg
soil. Firstly, all of the pots were divided into two groups on the basis
presence and/or not presence of PGPB and their humidity was daily
monitored by field capacity. To prepare PGPB suspension, the bacteriaMicrococcus yunnanensis was supplied from Department of soil
science, Shiraz University. The bacteria was incubated in nutrient broth
medium at 28°C for 24h on shaker continuously. When
sowing, seeds were treated with 1.0 ml bacteria suspension
(9×107 CFU ml-1) in the inoculated
groups. Water stress was applied to each pot at the levels of 75 and 50
% FC at the budding stage to full maturity. Camelina silique yield was
harvested at the maturity stage for analysis of seed quality and
biochemical responses in the treatment of water stress and PGPB.