No correlation between sodium currents versus content of Nav1.5 under estrogen treatment
Immunohistochemical staining performed to explore the effect of E2 on protein content of Nav1.5 revealed that E2 at 1 nmol/L decreased whereas 100 nmol/L increased the Nav1.5 protein staining signal (Supplemental Figure 6A and B; Supplemental Table 9). To determine the correlation between content of Nav1.5 and sodium currents, Pearson Correlation Coefficient was conducted. The results showed that there was no significant relationship between the effect of E2 on Nav1.5 content and currents (Supplemental Figure 6A, B, E and F). However, after ISO stimulation, E2 at all concentrations reversed the increase in the content of Nav1.5 induced by ISO (Supplemental Figure 6A, C and D; Supplemental Table 9). Pearson Correlation analysis demonstrated no association between the influence of E2 on Nav1.5 content and currents after ISO treatment (Supplemental Figure 6A, C-F).