Establishment of a cardiac stress model in hiPSC-CMs
By using protocols of modeling cardiac stress of our lab, together with methods described previously (Borchert et al., 2017; Zhao et al., 2018), 10 μmol/L ISO was used as the stress stimuli. In summary, hiPSC-CMs were incubated with 10 μmol/L ISO for 30 min to simulate cardiac stress.