Materials
Isoprenaline hydrochloride (ISO, Sigma, USA, Lot # WXBC9656V) was
dissolved in 0.01 mol/L HCl solution (36~38%), and then
PBS solution was added to prepare 10-3 mol/L ISO
mother liquor. β-Estradiol (E2, Sigma, USA, Lot # SLBT2822) was
dissolved in DMSO solution (the concentration of DMSO in extracellular
fluid was not higher than 0.1%) to prepare 2×10-1mol/L E2 mother liquor. G1 (Cayman, USA, Lot # 0459034-3) and G15
(Cayman, USA, Lot # 0458784-13) were dissolved in DMSO solution, and
then PBS solution was added to prepare G1 mother liquor and G15 mother
liquor respectively. These liquids were sub packed and stored in dark at
negative 20℃ and dilute to the desired concentration when used.