Materials
Isoprenaline hydrochloride (ISO, Sigma, USA, Lot # WXBC9656V) was dissolved in 0.01 mol/L HCl solution (36~38%), and then PBS solution was added to prepare 10-3 mol/L ISO mother liquor. β-Estradiol (E2, Sigma, USA, Lot # SLBT2822) was dissolved in DMSO solution (the concentration of DMSO in extracellular fluid was not higher than 0.1%) to prepare 2×10-1mol/L E2 mother liquor. G1 (Cayman, USA, Lot # 0459034-3) and G15 (Cayman, USA, Lot # 0458784-13) were dissolved in DMSO solution, and then PBS solution was added to prepare G1 mother liquor and G15 mother liquor respectively. These liquids were sub packed and stored in dark at negative 20℃ and dilute to the desired concentration when used.