No correlation between sodium currents versus content of
Nav1.5 under estrogen treatment
Immunohistochemical staining performed to explore the effect of E2 on
protein content of Nav1.5 revealed that E2 at 1 nmol/L
decreased whereas 100 nmol/L increased the Nav1.5
protein staining signal (Supplemental Figure 6A and B; Supplemental
Table 9). To determine the correlation between content of
Nav1.5 and sodium currents, Pearson Correlation
Coefficient was conducted. The results showed that there was no
significant relationship between the effect of E2 on
Nav1.5 content and currents (Supplemental Figure 6A, B,
E and F). However, after ISO stimulation, E2 at all concentrations
reversed the increase in the content of Nav1.5 induced
by ISO (Supplemental Figure 6A, C and D; Supplemental Table 9). Pearson
Correlation analysis demonstrated no association between the influence
of E2 on Nav1.5 content and currents after ISO treatment
(Supplemental Figure 6A, C-F).