Supplemental
Figure 2 ISO elevates sodium currents and alteresI Na kinetics as well as action potential
parameters in hiPSC-CMs. (A) Average I NaLdensity. (B) Current-voltage curve of the sodium channel. (C) PeakI Na density. (D) Time constant of recovery from
inactivation curve of the sodium channel. (E) τ value of recovery from
inactivation. (F) Activation and inactivation curve of the sodium
channel of hiPSC-CMs. (G-J) Values for k and V1/2of (in) activation. n=17-37 cells, from 9-11 petri dishes. (K)
Representative single AP and slope curve of hiPSC-CMs. (L-Q) Values for
APA, RMP, dV /dt max, APD10,
APD50 and APD90. n=20-34 cells, from 4
petri dishes, separately. An
unpaired t test was used. Welch’s correction was selectively used in
cases of uneven variance. *p < 0.05, **p< 0.01, ***p < 0.001, ****p< 0.0001. ISO, isoproterenol; I NaL,
late sodium current; I Na, sodium current;k of (in) activation, slope factor of (in) activation;
V1/2 of (in) activation, half-voltage of (in)
activation; τ, time constant of recovery from inactivation; APA, action
potential amplitude; RMP, resting membrane potential;
dV /dt max, maximal action potential
upstroke velocity; APD10, 50, 90, action potential
duration at 10%, 50% and 90% repolarization.
pA, pico Ampere; pF, pico Farad.
Insets: voltage clamp protocols.