By downregulating MMP-9
FLS were left untreated (CON) or transfected with control lentivirus
(LV-Plvx) or LV-EBP50 for 72 h. Then the effect of EBP50 on migration
was detected with transwell Boyden chamber,The images are representative
of migration through the membrane after staining. Original
magnifification 200×(A,C). The effect of EBP50 on migration was detected
with transwell Boyden chamber,The images are representative of invasion
through the membrane after staining. Original magnifification
200×(B,D).The expression levels of β-catenin and E-cadherin were
determined by Western blotting(E). Band density of the specific protein
was analyzed with Quantity One image software (F). The expression levels
of MMP-2 and MMP-9 were determined by Western blotting(G). Band density
of the specific protein was analyzed with Quantity One image software
(H-I). Data are represented as mean±SEM of at least three independent
experiments.*P < 0.05, **P < 0.01,***P <
0.001 compared with the uninfected group.