Figure 1. Overexpression of EBP50 Suppresses the proliferation
of FLS.
FLS were left untreated (CON) or
transfected with control lentivirus (LV-Plvx) or LV-EBP50 for 72 h.The
mRNA levels of EBP50 were measured by real-time RT-PCR (A). The protein
levels of EBP50 were detected by Western blotting (B).Band density of
the specific protein was analyzed with Quantity One image software, and
the results were expressed as average density to β-actin (C).FLS were
left untreated (CON) or transfected with control lentivirus (LV-Plvx) or
LV-EBP50 for 48 h, Then were transfered seedind in 96-well plates
5×103/cells,after night,20 μL MTT solution (0.5 mg/mL)
was added in FLS culture mediumand incubated for 4 h in 5% CO2
atmosphere at 37℃. The culture mediumand in each well was discarded and
added 150 μL DMSO. The absorption of each well was measured at 490
nm.(D). The protein levels of LC3 were detected by Western blotting
(E).Band density of the specific protein was analyzed with Quantity One
image software, and the results were expressed as average density to
β-actin (F).Data are represented as mean±SEM of at least three
independent experiments.*P < 0.05, **P < 0.01,***P
< 0.001 compared with the uninfected group.