Platelet function.
The data obtained in the PFA-100 study are shown in Table 4 (suppl). No statistically significant differences were observed between the groups. Similarly. when platelet aggregation function was analyzed with Chronolog, there were no significant differences between groups (suppl, Table 5). Equally, after analysing platelet function in complete blood with the Multiplate technique, no significant differences were found among groups (suppl, Table 6), except for a lower aggregation percentage in patients after the infusion with FVIII and the controls, when TRAP was used. Finally, the aggregation study and platelet activation markers expression, platelet-leukocyte aggregates and tissue factor exposure performed by flow cytometric analysis did not reveal any significant differences between groups (suppl, Table 7). Regarding calcium levels, both agonists, TRAP and ADP, elevated cytoplasmic calcium in all three groups but there were no significant differences between them (Figure 1). Although calcium release was lower in patients when TRAP was used, the analysis of the area under the curve of the calcium responses gave no statistical significance (Figure 2).