Platelet function.
The data obtained in the PFA-100 study are shown in Table 4 (suppl). No
statistically significant differences were observed between the groups.
Similarly. when platelet aggregation function was analyzed with
Chronolog, there were no significant differences between groups (suppl,
Table 5). Equally, after analysing platelet function in complete blood
with the Multiplate technique, no significant differences were found
among groups (suppl, Table 6), except for a lower aggregation percentage
in patients after the infusion with FVIII and the controls, when TRAP
was used. Finally, the aggregation study and platelet activation markers
expression, platelet-leukocyte aggregates and tissue factor exposure
performed by flow cytometric analysis did not reveal any significant
differences between groups (suppl, Table 7). Regarding calcium levels,
both agonists, TRAP and ADP, elevated cytoplasmic calcium in all three
groups but there were no significant differences between them (Figure
1). Although calcium release was lower in patients when TRAP was used,
the analysis of the area under the curve of the calcium responses gave
no statistical significance (Figure 2).