Figure 1- Circadian variation in 191 naturally occurring
accessions from Sweden
Delayed fluorescence rhythms were characterised by period, phase and RAE
and show significant variation (A-C). Colours represent the 10 most
extreme accessions for each phenotype which we use as tail groups for
further analysis. Some accessions belong to multiple tail groups as is
shown in D. This reflects the strong correlation between circadian
characteristics (See Supplementary Figure 4). Number of individual wells
contributing to the mean of each accession ranged from 4 to 18, with
each well representing the mean rhythms of approximately 15 seedlings.
Figure 2- Period phenotypes are linked by both the geography and
the genetic relatedness of accessions across Sweden
Figure A shows the location of origin of the 10 accessions with the
shortest (red triangles) and longest (blue triangles) periods. The
longest periods are found near the city of Malmo where day length and
temperature fluctuates less throughout the year than in Ornskoldsvik
where periods tend to be shorter (B,C). Day-length and temperature
averages were downloaded from timeanddate.com and are based on
predictions for 2019 (Thorsen, 1995).
PC analysis revealed a sub-clade within the Southern Swedish accessions;
PC.C coloured light blue in plots D-F. This was used to distinguish
accessions with significantly longer period phenotype than in the other
PC groups; PC.A which represents the remaining southern accessions in
yellow and PC.B which represents the northern accessions in red. Map
figures were created using the ggmaps package in R using Google maps
(accessed 2018) (Kahle & Wickham, 2013).