Assay with recombinant P450 isoforms
Seventeen cDNA-expressed human P450 isoforms, including CYP1A1, CYP1A2, CYP1B1, CYP2A6, CYP2B6, CYP2C19, CYP2C8, CYP2C9, CYP2D6, CYP2E1, CYP2J2, CYP3A4, CYP3A5, CYP3A7, CYP4F2, CYP4F3 and CYP46A1 were used. Rivaroxaban (120 μM) was incubated with recombinant CYPs at 37 °C for 60 minutes to estimate the participation of the CYP isoforms. Results were from at least three experiments carried out in duplicate. The quantitative determination of metabolite concentration was as described above.