Materials and chemicals
Rivaroxaban, sunitinib and NADPH were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. Imatinib and gefitinib were obtained from Sigma-Aldrich (Missouri, USA). cDNA-expressed recombinant human CYP3A4 was purchased from Cypex Ltd (Dundee, UK). Pooled human liver microsomes (HLM) and recombinant human CYP2J2 were purchased from BioreclamationIVT (Maryland, USA). All analytical reagent grade and HPLC grade solvents were from Tedia, Inc. (Ohio, USA). High-performance liquid chromatography (HPLC) was performed using an Agilent MSD/MS system controller, two 1260 series pumps, a 1200 series autosampler and a 1200 series variable wavelength detector.