Materials and chemicals
Rivaroxaban, sunitinib and NADPH were purchased from Shanghai Yuanye
Bio-Technology Co., Ltd. Imatinib and gefitinib were obtained from
Sigma-Aldrich (Missouri, USA). cDNA-expressed recombinant human CYP3A4
was purchased from Cypex Ltd (Dundee, UK). Pooled human liver microsomes
(HLM) and recombinant human CYP2J2 were purchased from
BioreclamationIVT
(Maryland, USA). All analytical reagent grade and HPLC grade solvents
were from Tedia, Inc. (Ohio, USA). High-performance liquid
chromatography (HPLC) was performed using an Agilent MSD/MS system
controller, two 1260 series pumps, a 1200 series autosampler and a 1200
series variable wavelength detector.