Statistical model
Statistical analysis and the relative graphs were made using
GraphPadPrism 6 (GraphPad Software Inc., La Jolla, CA, USA).
The statistical significance of the cytofluorimetric analyses were
assessed used using Student’s t-test, the number of single cell events
analyzed was never less than 9,980 cells out of 10,000 events at the
outset. The significance threshold was set p -value ≤0.05
(significant), p -value ≤0.01 (highly significant), andp -value ≤0.001 (extremely significant) (Bongiorno, Musso et
al., 2020). The evaluation of the MTT assay statistical significance
was assessed using ANOVA and Sidak’s multiple comparisons test (post hoc
test). The expression analysis statistical significance was assessed
using Ordinary one-way ANOVA and Bonferroni’s Multiple Comparison Test.