Eukaryotic cell culture preparation
Infection experiments were performed on the human osteosarcoma cell line
MG-63 (ATCC® CRL- 1427™, Standards Development
Organization, LGC Standards S.r.l.), as previously described (Bongiorno,
Musso et al ., 2020).
A single 6-well plate was used for the Imaging Flow Cytometry (IFC), a
single 6-well plate was used for RNA extraction and a 96-well plate was
used for the evaluation of eukaryotic cellular metabolism. All
experiments were performed twice in triplicate.