Genotypic characterization of Mycobacterium bovisisolates from dairy cattle
Elizabeth Hortêncio de Melo 1*, Harrison Magdinier Gomes2, Ícaro Rodrigues dos Santos2, José Augusto Bastos Afonso3, Marília Masello Junqueira Franco4, Hélio Langoni4, Antonio Carlos Paes4, Philip Noel Suffys2, Carla Lopes de Mendonça3
Elizabeth Hortêncio de Melo 1. Corresponding author: Elizabeth Hortêncio de Melo. Programa de Pós-Graduação em Medicina Veterinária, Universidade Federal Rural de Pernambuco, Rua Dom Manuel de Medeiros, S/N, Dois Irmãos. 52171-900. Brasil, Pernambuco. E-mail: elishmelo@hotmail.com
Harrison Magdinier Gomes2. Laboratório de Biologia Molecular Aplicada à Micobactérias, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brasil.
Ícaro Rodrigues dos Santos2. Laboratório de Biologia Molecular Aplicada à Micobactérias, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brasil.
José Augusto Bastos Afonso3. Clínica de Bovinos, Campus Garanhuns/UFRPE, Pernambuco, Brasil.
Marília Masello Junqueira Franco4. Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, UNESP, Botucatu, Brasil.
Helio Langoni4. Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, UNESP, Botucatu, Brasil.
Antonio Carlos Paes4. Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, UNESP, Botucatu, Brasil.
Philip Noel Suffys2. Laboratório de Biologia Molecular Aplicada à Micobactérias, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brasil.
Carla Lopes de Mendonça3. Clínica de Bovinos, Campus Garanhuns/UFRPE, Pernambuco, Brasil.
ABSTRACT
Molecular diagnosis of bovine tuberculosis plays an essential role in the epidemiological knowledge of the disease. Bovine tuberculosis caused by Mycobacterium bovis represents a risk to human health. This study aimed to perform the genotypic characterization of M. bovisisolated from bovines diagnosed as tuberculosis from dairy herds in the state of Pernambuco, Brazil. Granulomas from 30 bovines were sent for microbiological culture and colonies compatible withMycobacterium spp were obtained in at least one culture from 17/30 granulomas. All isolates were confirmed to be M. tuberculosis bovis by spoligotyping and 24loci MIRU-VNTR typing. While spoligotyping characterized the isolates as SB0121, SB0295, SB0852, SB0120 and an unclassified genotype, 24lociMIRU-VNTR rendered two clusters of two isolates each and 13 unique profiles. Loci ETR-A showed higher discriminatory power, andloci (ETR-B, ETR-C, MIRU16, MIRU27 and QUB26) showed moderate allelic diversity. This is the first study on genetic variability of the infectious agent cause of bovine TB in Pernambuco and demonstrates variability of strains in the state. Thus, it corroborates the importance of this microorganism as agent of bovine tuberculosis and its zoonotic potential, being this epidemiological tool determinant in the rigor of the sanitary practices of disease control in dairy herds.
Keywords : bovine tuberculosis, genotypic, Mycobacterium bovis .